Rebaudioside A Enhancement Series IV-IV-B

Stevia rebaudiana

Stevia

Tier IVRebaudioside A Enhancement Series

Target Trait

4.63×

fold-change

RebA yield fold-change

Technical Specifications

SKU

SS-F-SWT-232

Genomic Edits

GC Content

45.9%

Construct Length

4,308 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

463

Pathway Edits

UGT76G1 overexpression via strong 35S promoter for C-13 stevioside glucosylation
UGT74G1 upregulation for C-19 glucosylation step completion toward rebaudioside A
DXS overexpression to enhance MEP pathway flux into IPP and DMAPP precursors
KAH cytochrome P450 optimization for improved ent-kaurenoic acid hydroxylation efficiency
CDPG co-expression for ent-copalyl diphosphate precursor amplification
KO upregulation for ent-kaurene oxidation step enhancement toward steviol
RNAi cassette suppressing stevioside diversion pathway to maximize RebA-directed flux

Key Interventions

UGT76G1 glycosyltransferase overexpression for primary RebA glucosylation stepDXS-driven MEP pathway flux enhancement for steviol precursor supplyUGT74G1-mediated C-19 glucosylation for complete rebaudioside A assembly

IFI Subscale Profile

IFI Profile

IFI-S

463

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance variant: regulatory pre-submission RebA metabolite documentation for clean-label sweetener applications with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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