IFI-S · Rebaudioside A Enhancement Series

Specimen SS-F-SWT-229 // SWEET · IFI-S

Rebaudioside A Enhancement Series IV-PR-A

Stevia rebaudiana

Stevia

Tier IVRebaudioside A Enhancement Series

Target Trait

4.63×

fold-change

RebA yield fold-change

Technical Specifications

SKU

SS-F-SWT-229

Genomic Edits

8

GC Content

49.3%

Construct Length

4,257 bp

Promoter System

35S CaMV promoter

Expression System

PEG-mediated protoplast transfection

Host Strain

PEG/calcium transfection

Receptor Target

TAS1R2/TAS1R3

IFI Score

463

Pathway Edits

  • UGT76G1 overexpression via strong 35S promoter for C-13 stevioside glucosylation
  • UGT74G1 upregulation for C-19 glucosylation step completion toward rebaudioside A
  • DXS overexpression to enhance MEP pathway flux into IPP and DMAPP precursors
  • KAH cytochrome P450 optimization for improved ent-kaurenoic acid hydroxylation efficiency
  • CDPG co-expression for ent-copalyl diphosphate precursor amplification
  • KO upregulation for ent-kaurene oxidation step enhancement toward steviol
  • RNAi cassette suppressing stevioside diversion pathway to maximize RebA-directed flux

Key Interventions

UGT76G1 glycosyltransferase overexpression for primary RebA glucosylation stepDXS-driven MEP pathway flux enhancement for steviol precursor supplyUGT74G1-mediated C-19 glucosylation for complete rebaudioside A assembly

IFI Subscale Profile

IFI Profile

IFI-S
463
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

regulatory pre-submission RebA metabolite documentation for clean-label sweetener applications

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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