IFI-S · Rebaudioside A Enhancement Series
Specimen SS-F-SWT-221 // SWEET · IFI-S
Rebaudioside A Enhancement Series III-PR-A
Stevia rebaudiana
Stevia
Tier IIIRebaudioside A Enhancement Series
Target Trait
3.75×
fold-change
RebA yield fold-change
Technical Specifications
SKU
SS-F-SWT-221
Genomic Edits
7
GC Content
47.6%
Construct Length
3,910 bp
Promoter System
35S CaMV promoter
Expression System
PEG-mediated protoplast transfection
Host Strain
PEG/calcium transfection
Receptor Target
TAS1R2/TAS1R3
IFI Score
375
Pathway Edits
- UGT76G1 overexpression via strong 35S promoter for C-13 stevioside glucosylation
- UGT74G1 upregulation for C-19 glucosylation step completion toward rebaudioside A
- DXS overexpression to enhance MEP pathway flux into IPP and DMAPP precursors
- KAH cytochrome P450 optimization for improved ent-kaurenoic acid hydroxylation efficiency
- CDPG co-expression for ent-copalyl diphosphate precursor amplification
- KO upregulation for ent-kaurene oxidation step enhancement toward steviol
Key Interventions
UGT76G1 glycosyltransferase overexpression for primary RebA glucosylation stepDXS-driven MEP pathway flux enhancement for steviol precursor supplyUGT74G1-mediated C-19 glucosylation for complete rebaudioside A assembly
IFI Subscale Profile
IFI Profile
IFI-S375
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
commercial rebaudioside A platform for high-purity steviol glycoside ingredient supply
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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