ABK Tri-Axis Balanced Series V-IV

Vitis vinifera

Grape

Tier VABK Tri-Axis Balanced Series

Target Trait

2.5×

fold-change

Astringency+Bitter-Attenuation+Kokumi balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-02724

Genomic Edits

GC Content

43.9%

Construct Length

6,401 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis + TAS2R38 (suppression) + CaSR

IFI Score

250

Pathway Edits

Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
TAS2R38 (suppression) pathway primary gene cluster for bitter-attenuation (IFI-B) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent ABK pathway operation
Cross-pathway regulatory circuit for balanced ABK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: Polyphenol-astringency axis, TAS2R38 (suppression), and CaSR pathways simultaneously engineeredBalanced tri-axis expression strategy (ABK) with cross-pathway flux managementCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier V ABK triple-axis construct for advanced multi-modal sensory architecture combining astringency, bitter-attenuation, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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