USK Tri-Axis Dominant-Primary Series III-IV

Solanum lycopersicum

Tomato

Tier IIIUSK Tri-Axis Dominant-Primary Series

Target Trait

1.95×

fold-change

Umami-dominant tri-axis S+K modulated fold-change

Technical Specifications

SKU

SS-F-M3-02539

Genomic Edits

GC Content

46.5%

Construct Length

5,652 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

mGluR1 + TAS1R2/TAS1R3 + CaSR

IFI Score

195

Pathway Edits

mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
TAS1R2/TAS1R3 pathway primary gene cluster for sweet (IFI-S) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent USK pathway operation
Cross-pathway regulatory circuit for umami-dominant USK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: mGluR1, TAS1R2/TAS1R3, and CaSR pathways simultaneously engineeredUmami-dominant tri-axis expression strategy (USK) with cross-pathway flux managementCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III USK triple-axis construct for advanced multi-modal sensory architecture combining umami, sweet, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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