CUS Tri-Axis Dominant-Primary Series IV-IV

Mentha × piperita

Peppermint

Tier IVCUS Tri-Axis Dominant-Primary Series

Target Trait

2.38×

fold-change

Cooling-dominant tri-axis U+S modulated fold-change

Technical Specifications

SKU

SS-F-M3-01894

Genomic Edits

GC Content

45.4%

Construct Length

6,114 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPM8 + mGluR1 + TAS1R2/TAS1R3

IFI Score

238

Pathway Edits

TRPM8 pathway primary gene cluster for cooling (IFI-C) tri-axis component
mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
TAS1R2/TAS1R3 pathway primary gene cluster for sweet (IFI-S) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent CUS pathway operation
Cross-pathway regulatory circuit for cooling-dominant CUS triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPM8, mGluR1, and TAS1R2/TAS1R3 pathways simultaneously engineeredCooling-dominant tri-axis expression strategy (CUS) with cross-pathway flux managementCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV CUS triple-axis construct for advanced multi-modal sensory architecture combining cooling, umami, and sweet axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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