PBK Tri-Axis Balanced Series V-IV

Brassica nigra

Black Mustard

Tier VPBK Tri-Axis Balanced Series

Target Trait

2.5×

fold-change

Sharp+Bitter-Attenuation+Kokumi balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-01774

Genomic Edits

GC Content

45.4%

Construct Length

6,651 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + TAS2R38 (suppression) + CaSR

IFI Score

250

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
TAS2R38 (suppression) pathway primary gene cluster for bitter-attenuation (IFI-B) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PBK pathway operation
Cross-pathway regulatory circuit for balanced PBK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, TAS2R38 (suppression), and CaSR pathways simultaneously engineeredBalanced tri-axis expression strategy (PBK) with cross-pathway flux managementCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier V PBK triple-axis construct for advanced multi-modal sensory architecture combining sharp, bitter-attenuation, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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