PBK Tri-Axis Balanced Series IV-PR

Brassica nigra

Black Mustard

Tier IVPBK Tri-Axis Balanced Series

Target Trait

2.13×

fold-change

Sharp+Bitter-Attenuation+Kokumi balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-01768

Genomic Edits

GC Content

45.2%

Construct Length

5,740 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + TAS2R38 (suppression) + CaSR

IFI Score

213

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
TAS2R38 (suppression) pathway primary gene cluster for bitter-attenuation (IFI-B) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PBK pathway operation
Cross-pathway regulatory circuit for balanced PBK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, TAS2R38 (suppression), and CaSR pathways simultaneously engineeredBalanced tri-axis expression strategy (PBK) with cross-pathway flux managementPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PBK triple-axis construct for advanced multi-modal sensory architecture combining sharp, bitter-attenuation, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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