PAK Tri-Axis Dominant-Primary Series III-PR

Brassica nigra

Black Mustard

Tier IIIPAK Tri-Axis Dominant-Primary Series

Target Trait

1.95×

fold-change

Sharp-dominant tri-axis A+K modulated fold-change

Technical Specifications

SKU

SS-F-M3-01638

Genomic Edits

GC Content

45.3%

Construct Length

5,633 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + Polyphenol-astringency axis + CaSR

IFI Score

195

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PAK pathway operation
Cross-pathway regulatory circuit for sharp-dominant PAK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, Polyphenol-astringency axis, and CaSR pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PAK) with cross-pathway flux managementPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III PAK triple-axis construct for advanced multi-modal sensory architecture combining sharp, astringency, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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