PAK Tri-Axis Balanced Series IV-BL

Brassica nigra

Black Mustard

Tier IVPAK Tri-Axis Balanced Series

Target Trait

2.13×

fold-change

Sharp+Astringency+Kokumi balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-01617

Genomic Edits

GC Content

48.2%

Construct Length

5,871 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + Polyphenol-astringency axis + CaSR

IFI Score

213

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PAK pathway operation
Cross-pathway regulatory circuit for balanced PAK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, Polyphenol-astringency axis, and CaSR pathways simultaneously engineeredBalanced tri-axis expression strategy (PAK) with cross-pathway flux managementBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PAK triple-axis construct for advanced multi-modal sensory architecture combining sharp, astringency, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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