PAS Tri-Axis Dominant-Primary Series IV-AG

Brassica nigra

Black Mustard

Tier IVPAS Tri-Axis Dominant-Primary Series

Target Trait

2.38×

fold-change

Sharp-dominant tri-axis A+S modulated fold-change

Technical Specifications

SKU

SS-F-M3-01541

Genomic Edits

GC Content

47.6%

Construct Length

6,007 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPA1 + Polyphenol-astringency axis + TAS1R2/TAS1R3

IFI Score

238

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
TAS1R2/TAS1R3 pathway primary gene cluster for sweet (IFI-S) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PAS pathway operation
Cross-pathway regulatory circuit for sharp-dominant PAS triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, Polyphenol-astringency axis, and TAS1R2/TAS1R3 pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PAS) with cross-pathway flux managementT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PAS triple-axis construct for advanced multi-modal sensory architecture combining sharp, astringency, and sweet axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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