IFI-M · PAS Tri-Axis Balanced Series

Specimen SS-F-M3-01518 // MULTI-AXIS · IFI-M

PAS Tri-Axis Balanced Series IV-PR

Brassica nigra

Black Mustard

Tier IVPAS Tri-Axis Balanced Series

Target Trait

2.13×

fold-change

Sharp+Astringency+Sweet balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-01518

Genomic Edits

10

GC Content

45.3%

Construct Length

5,790 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + Polyphenol-astringency axis + TAS1R2/TAS1R3

IFI Score

213

Pathway Edits

  • TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
  • Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
  • TAS1R2/TAS1R3 pathway primary gene cluster for sweet (IFI-S) tri-axis component
  • Tri-axis metabolic flux partitioning hub for concurrent PAS pathway operation
  • Cross-pathway regulatory circuit for balanced PAS triple-axis expression
  • Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, Polyphenol-astringency axis, and TAS1R2/TAS1R3 pathways simultaneously engineeredBalanced tri-axis expression strategy (PAS) with cross-pathway flux managementPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV PAS triple-axis construct for advanced multi-modal sensory architecture combining sharp, astringency, and sweet axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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