PUK Tri-Axis Dominant-Primary Series IV-PR

Brassica nigra

Black Mustard

Tier IVPUK Tri-Axis Dominant-Primary Series

Target Trait

2.38×

fold-change

Sharp-dominant tri-axis U+K modulated fold-change

Technical Specifications

SKU

SS-F-M3-01493

Genomic Edits

GC Content

46.8%

Construct Length

5,995 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + mGluR1 + CaSR

IFI Score

238

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PUK pathway operation
Cross-pathway regulatory circuit for sharp-dominant PUK triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, mGluR1, and CaSR pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PUK) with cross-pathway flux managementPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier IV PUK triple-axis construct for advanced multi-modal sensory architecture combining sharp, umami, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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