IFI-M · PUB Tri-Axis Dominant-Primary Series

Specimen SS-F-M3-01428 // MULTI-AXIS · IFI-M

PUB Tri-Axis Dominant-Primary Series I-PR

Brassica nigra

Black Mustard

Tier IPUB Tri-Axis Dominant-Primary Series

Target Trait

1.1×

fold-change

Sharp-dominant tri-axis U+B modulated fold-change

Technical Specifications

SKU

SS-F-M3-01428

Genomic Edits

6

GC Content

43.4%

Construct Length

4,249 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1 + mGluR1 + TAS2R38 (suppression)

IFI Score

110

Pathway Edits

  • TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
  • mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
  • TAS2R38 (suppression) pathway primary gene cluster for bitter-attenuation (IFI-B) tri-axis component
  • Tri-axis metabolic flux partitioning hub for concurrent PUB pathway operation
  • Cross-pathway regulatory circuit for sharp-dominant PUB triple-axis expression
  • Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, mGluR1, and TAS2R38 (suppression) pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PUB) with cross-pathway flux managementPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier I PUB triple-axis construct for advanced multi-modal sensory architecture combining sharp, umami, and bitter-attenuation axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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