PUB Tri-Axis Balanced Series II-IV

Brassica nigra

Black Mustard

Tier IIPUB Tri-Axis Balanced Series

Target Trait

1.38×

fold-change

Sharp+Umami+Bitter-Attenuation balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-01409

Genomic Edits

GC Content

47.8%

Construct Length

4,605 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + mGluR1 + TAS2R38 (suppression)

IFI Score

138

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
TAS2R38 (suppression) pathway primary gene cluster for bitter-attenuation (IFI-B) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PUB pathway operation
Cross-pathway regulatory circuit for balanced PUB triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, mGluR1, and TAS2R38 (suppression) pathways simultaneously engineeredBalanced tri-axis expression strategy (PUB) with cross-pathway flux managementCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II PUB triple-axis construct for advanced multi-modal sensory architecture combining sharp, umami, and bitter-attenuation axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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