PUS Tri-Axis Dominant-Primary Series I-BL

Brassica nigra

Black Mustard

Tier IPUS Tri-Axis Dominant-Primary Series

Target Trait

1.1×

fold-change

Sharp-dominant tri-axis U+S modulated fold-change

Technical Specifications

SKU

SS-F-M3-01377

Genomic Edits

GC Content

48.2%

Construct Length

4,180 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + mGluR1 + TAS1R2/TAS1R3

IFI Score

110

Pathway Edits

TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
TAS1R2/TAS1R3 pathway primary gene cluster for sweet (IFI-S) tri-axis component
Tri-axis metabolic flux partitioning hub for concurrent PUS pathway operation
Cross-pathway regulatory circuit for sharp-dominant PUS triple-axis expression
Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, mGluR1, and TAS1R2/TAS1R3 pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PUS) with cross-pathway flux managementBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I PUS triple-axis construct for advanced multi-modal sensory architecture combining sharp, umami, and sweet axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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