IFI-M · PUA Tri-Axis Dominant-Primary Series

Specimen SS-F-M3-01332 // MULTI-AXIS · IFI-M

PUA Tri-Axis Dominant-Primary Series II-BL

Brassica nigra

Black Mustard

Tier IIPUA Tri-Axis Dominant-Primary Series

Target Trait

1.52×

fold-change

Sharp-dominant tri-axis U+A modulated fold-change

Technical Specifications

SKU

SS-F-M3-01332

Genomic Edits

8

GC Content

44.6%

Construct Length

5,022 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1 + mGluR1 + Polyphenol-astringency axis

IFI Score

152

Pathway Edits

  • TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
  • mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
  • Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
  • Tri-axis metabolic flux partitioning hub for concurrent PUA pathway operation
  • Cross-pathway regulatory circuit for sharp-dominant PUA triple-axis expression
  • Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, mGluR1, and Polyphenol-astringency axis pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PUA) with cross-pathway flux managementBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II PUA triple-axis construct for advanced multi-modal sensory architecture combining sharp, umami, and astringency axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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