IFI-M · PUA Tri-Axis Balanced Series

Specimen SS-F-M3-01316 // MULTI-AXIS · IFI-M

PUA Tri-Axis Balanced Series IV-AG

Brassica nigra

Black Mustard

Tier IVPUA Tri-Axis Balanced Series

Target Trait

2.13×

fold-change

Sharp+Umami+Astringency balanced tri-axis fold-change

Technical Specifications

SKU

SS-F-M3-01316

Genomic Edits

10

GC Content

44.4%

Construct Length

5,852 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPA1 + mGluR1 + Polyphenol-astringency axis

IFI Score

213

Pathway Edits

  • TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
  • mGluR1 pathway primary gene cluster for umami (IFI-U) tri-axis component
  • Polyphenol-astringency axis pathway primary gene cluster for astringency (IFI-A) tri-axis component
  • Tri-axis metabolic flux partitioning hub for concurrent PUA pathway operation
  • Cross-pathway regulatory circuit for balanced PUA triple-axis expression
  • Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, mGluR1, and Polyphenol-astringency axis pathways simultaneously engineeredBalanced tri-axis expression strategy (PUA) with cross-pathway flux managementT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV PUA triple-axis construct for advanced multi-modal sensory architecture combining sharp, umami, and astringency axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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