IFI-M · PCK Tri-Axis Dominant-Primary Series

Specimen SS-F-M3-01299 // MULTI-AXIS · IFI-M

PCK Tri-Axis Dominant-Primary Series V-IV

Brassica nigra

Black Mustard

Tier VPCK Tri-Axis Dominant-Primary Series

Target Trait

2.8×

fold-change

Sharp-dominant tri-axis C+K modulated fold-change

Technical Specifications

SKU

SS-F-M3-01299

Genomic Edits

13

GC Content

46.5%

Construct Length

7,006 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1 + TRPM8 + CaSR

IFI Score

280

Pathway Edits

  • TRPA1 pathway primary gene cluster for sharp (IFI-P) tri-axis component
  • TRPM8 pathway primary gene cluster for cooling (IFI-C) tri-axis component
  • CaSR pathway primary gene cluster for kokumi (IFI-K) tri-axis component
  • Tri-axis metabolic flux partitioning hub for concurrent PCK pathway operation
  • Cross-pathway regulatory circuit for sharp-dominant PCK triple-axis expression
  • Cofactor regeneration system (NADPH, ATP, acetyl-CoA) supporting triple-axis concurrent production

Key Interventions

Triple-axis receptor co-activation: TRPA1, TRPM8, and CaSR pathways simultaneously engineeredSharp-dominant tri-axis expression strategy (PCK) with cross-pathway flux managementCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V PCK triple-axis construct for advanced multi-modal sensory architecture combining sharp, cooling, and kokumi axes. Suitable for ultra-premium ingredient formulation requiring certification across three simultaneous IFI receptor pathways.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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