IFI-M · BK Precision Dual-Axis Series

Specimen SS-F-M2-08399 // MULTI-AXIS · IFI-M

BK Precision Dual-Axis Series V-IV-B

Brassica oleracea

Broccoli

Tier VBK Precision Dual-Axis Series

Target Trait

5.04×

fold-change

Bitter-Attenuation+Kokumi precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-08399

Genomic Edits

13

GC Content

47.4%

Construct Length

6,278 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS2R38 (suppression) + CaSR

IFI Score

504

Pathway Edits

  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized B+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-B and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Bitter-Attenuation-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for B+K simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized BK spatial patterning
  • Chassis organism metabolic engineering for BK dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TAS2R38 (suppression) and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous bitter-attenuation and kokumi accumulation without competing pathway suppressionPrecision combinatorial regulation for BK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V BK dual-axis precision dual platform for food ingredient applications requiring verified combined bitter-attenuation+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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