IFI-M · BK Tissue-Specific Series

Specimen SS-F-M2-08331 // MULTI-AXIS · IFI-M

BK Tissue-Specific Series V-AG-C

Brassica oleracea

Broccoli

Tier VBK Tissue-Specific Series

Target Trait

3.5×

fold-change

Bitter-Attenuation+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-08331

Genomic Edits

11

GC Content

49.1%

Construct Length

5,562 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS2R38 (suppression) + CaSR

IFI Score

350

Pathway Edits

  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized B+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-B and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Bitter-Attenuation-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for B+K simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized BK spatial patterning
  • Protein scaffold engineering for B+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TAS2R38 (suppression) and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous bitter-attenuation and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for BK multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V BK dual-axis tissue-specific platform for food ingredient applications requiring verified combined bitter-attenuation+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10149 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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