BK Tissue-Specific Series I-BL-B

Brassica oleracea

Broccoli

Tier IBK Tissue-Specific Series

Target Trait

1.23×

fold-change

Bitter-Attenuation+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-08285

Genomic Edits

GC Content

48.5%

Construct Length

3,176 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS2R38 (suppression) + CaSR

IFI Score

123

Pathway Edits

TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized B+K dual-axis co-expression
Metabolic flux partitioning between IFI-B and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Bitter-Attenuation-Kokumi combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized TAS2R38 (suppression) and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous bitter-attenuation and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for BK multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I BK dual-axis tissue-specific platform for food ingredient applications requiring verified combined bitter-attenuation+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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