IFI-M · BK Sequential Integration Series

Specimen SS-F-M2-08202 // MULTI-AXIS · IFI-M

BK Sequential Integration Series IV-BL-C

Brassica oleracea

Broccoli

Tier IVBK Sequential Integration Series

Target Trait

3.69×

fold-change

Bitter-Attenuation+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-08202

Genomic Edits

11

GC Content

46.3%

Construct Length

5,538 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS2R38 (suppression) + CaSR

IFI Score

369

Pathway Edits

  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized B+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-B and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Bitter-Attenuation-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for B+K simultaneous accumulation
  • Cofactor balance optimization for B+K dual-pathway operation
  • Protein scaffold engineering for B+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TAS2R38 (suppression) and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous bitter-attenuation and kokumi accumulation without competing pathway suppressionSequential construct stacking for BK multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV BK dual-axis sequential integration platform for food ingredient applications requiring verified combined bitter-attenuation+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4149 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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