IFI-M · SK Tissue-Specific Series

Specimen SS-F-M2-08024 // MULTI-AXIS · IFI-M

SK Tissue-Specific Series IV-PR-B

Stevia rebaudiana

Stevia

Tier IVSK Tissue-Specific Series

Target Trait

2.67×

fold-change

Sweet+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-08024

Genomic Edits

9

GC Content

44.3%

Construct Length

4,752 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R2/TAS1R3 + CaSR

IFI Score

267

Pathway Edits

  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized S+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-S and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sweet-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for S+K simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized SK spatial patterning
  • Protein scaffold engineering for S+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for SK multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV SK dual-axis tissue-specific platform for food ingredient applications requiring verified combined sweet+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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