IFI-M · SK Flux-Balanced Series

Specimen SS-F-M2-07978 // MULTI-AXIS · IFI-M

SK Flux-Balanced Series V-IV-A

Stevia rebaudiana

Stevia

Tier VSK Flux-Balanced Series

Target Trait

4×

fold-change

Sweet+Kokumi flux-balanced output

Technical Specifications

SKU

SS-F-M2-07978

Genomic Edits

11

GC Content

44.9%

Construct Length

5,561 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3 + CaSR

IFI Score

400

Pathway Edits

  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized S+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-S and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sweet-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for S+K simultaneous accumulation
  • Cofactor balance optimization for S+K dual-pathway operation
  • Protein scaffold engineering for S+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and kokumi accumulation without competing pathway suppressionFlux-balanced gene dosing for SK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V SK dual-axis flux-balanced platform for food ingredient applications requiring verified combined sweet+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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