SK Flux-Balanced Series II-IV-A

Stevia rebaudiana

Stevia

Tier IISK Flux-Balanced Series

Target Trait

2.05×

fold-change

Sweet+Kokumi flux-balanced output

Technical Specifications

SKU

SS-F-M2-07942

Genomic Edits

GC Content

43.8%

Construct Length

3,936 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3 + CaSR

IFI Score

205

Pathway Edits

TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized S+K dual-axis co-expression
Metabolic flux partitioning between IFI-S and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sweet-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for S+K simultaneous accumulation
Cofactor balance optimization for S+K dual-pathway operation

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and kokumi accumulation without competing pathway suppressionFlux-balanced gene dosing for SK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II SK dual-axis flux-balanced platform for food ingredient applications requiring verified combined sweet+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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