IFI-M · SK Co-Expression Series

Specimen SS-F-M2-07841 // MULTI-AXIS · IFI-M

SK Co-Expression Series IV-BL-B

Stevia rebaudiana

Stevia

Tier IVSK Co-Expression Series

Target Trait

2.86×

fold-change

Sweet+Kokumi co-activation fold-change

Technical Specifications

SKU

SS-F-M2-07841

Genomic Edits

9

GC Content

45.8%

Construct Length

4,641 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS1R2/TAS1R3 + CaSR

IFI Score

286

Pathway Edits

  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized S+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-S and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sweet-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for S+K simultaneous accumulation
  • Cofactor balance optimization for S+K dual-pathway operation
  • Protein scaffold engineering for S+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and kokumi accumulation without competing pathway suppressionCo-expression vector design for SK multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV SK dual-axis co-expression platform for food ingredient applications requiring verified combined sweet+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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