IFI-M · SB Precision Dual-Axis Series

Specimen SS-F-M2-07774 // MULTI-AXIS · IFI-M

SB Precision Dual-Axis Series III-IV-A

Stevia rebaudiana

Stevia

Tier IIISB Precision Dual-Axis Series

Target Trait

3×

fold-change

Sweet+Bitter-Attenuation precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-07774

Genomic Edits

9

GC Content

45.4%

Construct Length

4,671 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3 + TAS2R38 (suppression)

IFI Score

300

Pathway Edits

  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized S+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-S and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sweet-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for S+B simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized SB spatial patterning
  • Chassis organism metabolic engineering for SB dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and bitter-attenuation accumulation without competing pathway suppressionPrecision combinatorial regulation for SB multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III SB dual-axis precision dual platform for food ingredient applications requiring verified combined sweet+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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