SB Tissue-Specific Series II-IV-A

Stevia rebaudiana

Stevia

Tier IISB Tissue-Specific Series

Target Trait

1.52×

fold-change

Sweet+Bitter-Attenuation tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-07702

Genomic Edits

GC Content

43.8%

Construct Length

3,296 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3 + TAS2R38 (suppression)

IFI Score

152

Pathway Edits

TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
Bidirectional promoter system for synchronized S+B dual-axis co-expression
Metabolic flux partitioning between IFI-S and IFI-B pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sweet-Bitter-Attenuation combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and bitter-attenuation accumulation without competing pathway suppressionTissue-specific dual expression for SB multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II SB dual-axis tissue-specific platform for food ingredient applications requiring verified combined sweet+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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