IFI-M · SB Flux-Balanced Series

Specimen SS-F-M2-07674 // MULTI-AXIS · IFI-M

SB Flux-Balanced Series V-BL-C

Stevia rebaudiana

Stevia

Tier VSB Flux-Balanced Series

Target Trait

5×

fold-change

Sweet+Bitter-Attenuation flux-balanced output

Technical Specifications

SKU

SS-F-M2-07674

Genomic Edits

13

GC Content

43.6%

Construct Length

6,153 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS1R2/TAS1R3 + TAS2R38 (suppression)

IFI Score

500

Pathway Edits

  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized S+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-S and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Sweet-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for S+B simultaneous accumulation
  • Cofactor balance optimization for S+B dual-pathway operation
  • Protein scaffold engineering for S+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and bitter-attenuation accumulation without competing pathway suppressionFlux-balanced gene dosing for SB multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V SB dual-axis flux-balanced platform for food ingredient applications requiring verified combined sweet+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10149 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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