SB Sequential Integration Series I-AG-A

Stevia rebaudiana

Stevia

Tier ISB Sequential Integration Series

Target Trait

1.3×

fold-change

Sweet+Bitter-Attenuation sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-07561

Genomic Edits

GC Content

45.8%

Construct Length

3,268 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R2/TAS1R3 + TAS2R38 (suppression)

IFI Score

130

Pathway Edits

TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
Bidirectional promoter system for synchronized S+B dual-axis co-expression
Metabolic flux partitioning between IFI-S and IFI-B pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sweet-Bitter-Attenuation combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and bitter-attenuation accumulation without competing pathway suppressionSequential construct stacking for SB multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I SB dual-axis sequential integration platform for food ingredient applications requiring verified combined sweet+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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