SB Co-Expression Series I-PR-B

Stevia rebaudiana

Stevia

Tier ISB Co-Expression Series

Target Trait

1.34×

fold-change

Sweet+Bitter-Attenuation co-activation fold-change

Technical Specifications

SKU

SS-F-M2-07508

Genomic Edits

GC Content

43.4%

Construct Length

3,167 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R2/TAS1R3 + TAS2R38 (suppression)

IFI Score

134

Pathway Edits

TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
Bidirectional promoter system for synchronized S+B dual-axis co-expression
Metabolic flux partitioning between IFI-S and IFI-B pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Sweet-Bitter-Attenuation combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized TAS1R2/TAS1R3 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous sweet and bitter-attenuation accumulation without competing pathway suppressionCo-expression vector design for SB multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I SB dual-axis co-expression platform for food ingredient applications requiring verified combined sweet+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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