IFI-M · AK Sequential Integration Series

Specimen SS-F-M2-07294 // MULTI-AXIS · IFI-M

AK Sequential Integration Series III-IV-A

Vitis vinifera

Grape

Tier IIIAK Sequential Integration Series

Target Trait

2.4×

fold-change

Astringency+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-07294

Genomic Edits

8

GC Content

45.4%

Construct Length

4,331 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis + CaSR

IFI Score

240

Pathway Edits

  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized A+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-A and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Astringency-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for A+K simultaneous accumulation
  • Cofactor balance optimization for A+K dual-pathway operation
  • Protein scaffold engineering for A+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and kokumi accumulation without competing pathway suppressionSequential construct stacking for AK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III AK dual-axis sequential integration platform for food ingredient applications requiring verified combined astringency+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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