AK Sequential Integration Series I-AG-A

Vitis vinifera

Grape

Tier IAK Sequential Integration Series

Target Trait

1.3×

fold-change

Astringency+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-07261

Genomic Edits

GC Content

47.6%

Construct Length

3,168 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis + CaSR

IFI Score

130

Pathway Edits

Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized A+K dual-axis co-expression
Metabolic flux partitioning between IFI-A and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Astringency-Kokumi combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and kokumi accumulation without competing pathway suppressionSequential construct stacking for AK multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I AK dual-axis sequential integration platform for food ingredient applications requiring verified combined astringency+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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