AK Co-Expression Series II-BL-B

Vitis vinifera

Grape

Tier IIAK Co-Expression Series

Target Trait

1.85×

fold-change

Astringency+Kokumi co-activation fold-change

Technical Specifications

SKU

SS-F-M2-07217

Genomic Edits

GC Content

48.2%

Construct Length

3,631 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis + CaSR

IFI Score

185

Pathway Edits

Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized A+K dual-axis co-expression
Metabolic flux partitioning between IFI-A and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Astringency-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for A+K simultaneous accumulation

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and kokumi accumulation without competing pathway suppressionCo-expression vector design for AK multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II AK dual-axis co-expression platform for food ingredient applications requiring verified combined astringency+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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