IFI-M · AB Precision Dual-Axis Series

Specimen SS-F-M2-07154 // MULTI-AXIS · IFI-M

AB Precision Dual-Axis Series II-AG-B

Vitis vinifera

Grape

Tier IIAB Precision Dual-Axis Series

Target Trait

2.52×

fold-change

Astringency+Bitter-Attenuation precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-07154

Genomic Edits

9

GC Content

43.6%

Construct Length

4,700 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis + TAS2R38 (suppression)

IFI Score

252

Pathway Edits

  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized A+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-A and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Astringency-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for A+B simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized AB spatial patterning
  • Chassis organism metabolic engineering for AB dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and bitter-attenuation accumulation without competing pathway suppressionPrecision combinatorial regulation for AB multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II AB dual-axis precision dual platform for food ingredient applications requiring verified combined astringency+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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