IFI-M · AB Sequential Integration Series

Specimen SS-F-M2-07004 // MULTI-AXIS · IFI-M

AB Sequential Integration Series IV-PR-B

Vitis vinifera

Grape

Tier IVAB Sequential Integration Series

Target Trait

3.3×

fold-change

Astringency+Bitter-Attenuation sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-07004

Genomic Edits

10

GC Content

43.9%

Construct Length

5,192 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis + TAS2R38 (suppression)

IFI Score

330

Pathway Edits

  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized A+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-A and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Astringency-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for A+B simultaneous accumulation
  • Cofactor balance optimization for A+B dual-pathway operation
  • Protein scaffold engineering for A+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and bitter-attenuation accumulation without competing pathway suppressionSequential construct stacking for AB multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV AB dual-axis sequential integration platform for food ingredient applications requiring verified combined astringency+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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