IFI-M · AS Precision Dual-Axis Series

Specimen SS-F-M2-06899 // MULTI-AXIS · IFI-M

AS Precision Dual-Axis Series V-IV-B

Vitis vinifera

Grape

Tier VAS Precision Dual-Axis Series

Target Trait

5.04×

fold-change

Astringency+Sweet precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-06899

Genomic Edits

13

GC Content

46.5%

Construct Length

6,178 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis + TAS1R2/TAS1R3

IFI Score

504

Pathway Edits

  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • Bidirectional promoter system for synchronized A+S dual-axis co-expression
  • Metabolic flux partitioning between IFI-A and IFI-S pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Astringency-Sweet combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for A+S simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized AS spatial patterning
  • Chassis organism metabolic engineering for AS dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and sweet accumulation without competing pathway suppressionPrecision combinatorial regulation for AS multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V AS dual-axis precision dual platform for food ingredient applications requiring verified combined astringency+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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