AS Tissue-Specific Series II-BL-A

Vitis vinifera

Grape

Tier IIAS Tissue-Specific Series

Target Trait

1.52×

fold-change

Astringency+Sweet tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-06796

Genomic Edits

GC Content

44.4%

Construct Length

3,294 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis + TAS1R2/TAS1R3

IFI Score

152

Pathway Edits

Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
Bidirectional promoter system for synchronized A+S dual-axis co-expression
Metabolic flux partitioning between IFI-A and IFI-S pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Astringency-Sweet combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and sweet accumulation without competing pathway suppressionTissue-specific dual expression for AS multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II AS dual-axis tissue-specific platform for food ingredient applications requiring verified combined astringency+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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