IFI-M · AS Sequential Integration Series

Specimen SS-F-M2-06714 // MULTI-AXIS · IFI-M

AS Sequential Integration Series V-BL-C

Vitis vinifera

Grape

Tier VAS Sequential Integration Series

Target Trait

4.38×

fold-change

Astringency+Sweet sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-06714

Genomic Edits

12

GC Content

43.6%

Construct Length

5,853 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis + TAS1R2/TAS1R3

IFI Score

438

Pathway Edits

  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
  • Bidirectional promoter system for synchronized A+S dual-axis co-expression
  • Metabolic flux partitioning between IFI-A and IFI-S pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Astringency-Sweet combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for A+S simultaneous accumulation
  • Cofactor balance optimization for A+S dual-pathway operation
  • Protein scaffold engineering for A+S enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and sweet accumulation without competing pathway suppressionSequential construct stacking for AS multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V AS dual-axis sequential integration platform for food ingredient applications requiring verified combined astringency+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10149 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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