AS Sequential Integration Series I-IV-A

Vitis vinifera

Grape

Tier IAS Sequential Integration Series

Target Trait

1.3×

fold-change

Astringency+Sweet sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-06670

Genomic Edits

GC Content

44.8%

Construct Length

3,121 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis + TAS1R2/TAS1R3

IFI Score

130

Pathway Edits

Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
TAS1R2/TAS1R3 pathway primary gene cluster overexpression for sweet axis activation
Bidirectional promoter system for synchronized A+S dual-axis co-expression
Metabolic flux partitioning between IFI-A and IFI-S pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Astringency-Sweet combinatorial gene regulation

Key Interventions

Dual-receptor co-activation via synchronized Polyphenol-astringency axis and TAS1R2/TAS1R3 pathway engineeringMetabolic flux partitioning strategy enabling simultaneous astringency and sweet accumulation without competing pathway suppressionSequential construct stacking for AS multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I AS dual-axis sequential integration platform for food ingredient applications requiring verified combined astringency+sweet sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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