UK Tissue-Specific Series III-IV-A

Solanum lycopersicum

Tomato

Tier IIIUK Tissue-Specific Series

Target Trait

1.95×

fold-change

Umami+Kokumi tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-06514

Genomic Edits

GC Content

43.6%

Construct Length

3,891 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

mGluR1 + CaSR

IFI Score

195

Pathway Edits

mGluR1 pathway primary gene cluster overexpression for umami axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized U+K dual-axis co-expression
Metabolic flux partitioning between IFI-U and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Umami-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for U+K simultaneous accumulation
Tissue-specific expression cassette deployment for optimized UK spatial patterning

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and kokumi accumulation without competing pathway suppressionTissue-specific dual expression for UK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier III UK dual-axis tissue-specific platform for food ingredient applications requiring verified combined umami+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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