IFI-M · UK Flux-Balanced Series

Specimen SS-F-M2-06440 // MULTI-AXIS · IFI-M

UK Flux-Balanced Series II-PR-B

Solanum lycopersicum

Tomato

Tier IIUK Flux-Balanced Series

Target Trait

2.3×

fold-change

Umami+Kokumi flux-balanced output

Technical Specifications

SKU

SS-F-M2-06440

Genomic Edits

8

GC Content

44.2%

Construct Length

4,382 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 + CaSR

IFI Score

230

Pathway Edits

  • mGluR1 pathway primary gene cluster overexpression for umami axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized U+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-U and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Umami-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for U+K simultaneous accumulation
  • Cofactor balance optimization for U+K dual-pathway operation
  • Protein scaffold engineering for U+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and kokumi accumulation without competing pathway suppressionFlux-balanced gene dosing for UK multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II UK dual-axis flux-balanced platform for food ingredient applications requiring verified combined umami+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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