UK Flux-Balanced Series I-BL-C

Solanum lycopersicum

Tomato

Tier IUK Flux-Balanced Series

Target Trait

1.75×

fold-change

Umami+Kokumi flux-balanced output

Technical Specifications

SKU

SS-F-M2-06426

Genomic Edits

GC Content

45.6%

Construct Length

3,933 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

mGluR1 + CaSR

IFI Score

175

Pathway Edits

mGluR1 pathway primary gene cluster overexpression for umami axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized U+K dual-axis co-expression
Metabolic flux partitioning between IFI-U and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Umami-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for U+K simultaneous accumulation
Cofactor balance optimization for U+K dual-pathway operation

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and kokumi accumulation without competing pathway suppressionFlux-balanced gene dosing for UK multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier I UK dual-axis flux-balanced platform for food ingredient applications requiring verified combined umami+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$449 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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