UK Sequential Integration Series V-AG-C

Solanum lycopersicum

Tomato

Tier VUK Sequential Integration Series

Target Trait

4.38×

fold-change

Umami+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-06411

Genomic Edits

GC Content

49.1%

Construct Length

5,902 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 + CaSR

IFI Score

438

Pathway Edits

mGluR1 pathway primary gene cluster overexpression for umami axis activation
CaSR pathway primary gene cluster overexpression for kokumi axis activation
Bidirectional promoter system for synchronized U+K dual-axis co-expression
Metabolic flux partitioning between IFI-U and IFI-K pathway branches via carbon flow optimization
Transcription factor co-binding site engineering for Umami-Kokumi combinatorial gene regulation
Cross-pathway feedback inhibition circuit removal for U+K simultaneous accumulation
Cofactor balance optimization for U+K dual-pathway operation
Protein scaffold engineering for U+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and kokumi accumulation without competing pathway suppressionSequential construct stacking for UK multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier V UK dual-axis sequential integration platform for food ingredient applications requiring verified combined umami+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$10149 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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