IFI-M · UK Sequential Integration Series

Specimen SS-F-M2-06397 // MULTI-AXIS · IFI-M

UK Sequential Integration Series IV-AG-A

Solanum lycopersicum

Tomato

Tier IVUK Sequential Integration Series

Target Trait

2.95×

fold-change

Umami+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-06397

Genomic Edits

9

GC Content

46.9%

Construct Length

4,693 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 + CaSR

IFI Score

295

Pathway Edits

  • mGluR1 pathway primary gene cluster overexpression for umami axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized U+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-U and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Umami-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for U+K simultaneous accumulation
  • Cofactor balance optimization for U+K dual-pathway operation
  • Protein scaffold engineering for U+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and kokumi accumulation without competing pathway suppressionSequential construct stacking for UK multi-axis integrationT-DNA binary vector 2A11 for stable genomic integration of multi-axis construct stack

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV UK dual-axis sequential integration platform for food ingredient applications requiring verified combined umami+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Multi-Axis · IFI-MAll Modalities