IFI-M · UB Tissue-Specific Series

Specimen SS-F-M2-06215 // MULTI-AXIS · IFI-M

UB Tissue-Specific Series III-IV-B

Solanum lycopersicum

Tomato

Tier IIIUB Tissue-Specific Series

Target Trait

2.18×

fold-change

Umami+Bitter-Attenuation tissue-specific dual expression

Technical Specifications

SKU

SS-F-M2-06215

Genomic Edits

8

GC Content

47.3%

Construct Length

4,388 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

mGluR1 + TAS2R38 (suppression)

IFI Score

218

Pathway Edits

  • mGluR1 pathway primary gene cluster overexpression for umami axis activation
  • TAS2R38 (suppression) pathway primary gene cluster overexpression for bitter-attenuation axis activation
  • Bidirectional promoter system for synchronized U+B dual-axis co-expression
  • Metabolic flux partitioning between IFI-U and IFI-B pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Umami-Bitter-Attenuation combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for U+B simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized UB spatial patterning
  • Protein scaffold engineering for U+B enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and TAS2R38 (suppression) pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and bitter-attenuation accumulation without competing pathway suppressionTissue-specific dual expression for UB multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III UB dual-axis tissue-specific platform for food ingredient applications requiring verified combined umami+bitter-attenuation sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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