IFI-M · UA Sequential Integration Series

Specimen SS-F-M2-05512 // MULTI-AXIS · IFI-M

UA Sequential Integration Series V-BL-A

Solanum lycopersicum

Tomato

Tier VUA Sequential Integration Series

Target Trait

3.5×

fold-change

Umami+Astringency sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-05512

Genomic Edits

10

GC Content

45.9%

Construct Length

5,059 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

mGluR1 + Polyphenol-astringency axis

IFI Score

350

Pathway Edits

  • mGluR1 pathway primary gene cluster overexpression for umami axis activation
  • Polyphenol-astringency axis pathway primary gene cluster overexpression for astringency axis activation
  • Bidirectional promoter system for synchronized U+A dual-axis co-expression
  • Metabolic flux partitioning between IFI-U and IFI-A pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Umami-Astringency combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for U+A simultaneous accumulation
  • Cofactor balance optimization for U+A dual-pathway operation
  • Protein scaffold engineering for U+A enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized mGluR1 and Polyphenol-astringency axis pathway engineeringMetabolic flux partitioning strategy enabling simultaneous umami and astringency accumulation without competing pathway suppressionSequential construct stacking for UA multi-axis integrationBiolistic co-bombardment of multi-axis transgene cassettes for high-copy integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V UA dual-axis sequential integration platform for food ingredient applications requiring verified combined umami+astringency sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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