IFI-M · CK Precision Dual-Axis Series

Specimen SS-F-M2-05387 // MULTI-AXIS · IFI-M

CK Precision Dual-Axis Series IV-IV-B

Mentha × piperita

Peppermint

Tier IVCK Precision Dual-Axis Series

Target Trait

4.2×

fold-change

Cooling+Kokumi precision dual-axis IFI score

Technical Specifications

SKU

SS-F-M2-05387

Genomic Edits

12

GC Content

47%

Construct Length

5,863 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPM8 + CaSR

IFI Score

420

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized C+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+K simultaneous accumulation
  • Tissue-specific expression cassette deployment for optimized CK spatial patterning
  • Chassis organism metabolic engineering for CK dual-output receptor target optimization

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and kokumi accumulation without competing pathway suppressionPrecision combinatorial regulation for CK multi-axis integrationCell-free co-translation of multi-axis enzyme sets for in-vitro biochemical validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV CK dual-axis precision dual platform for food ingredient applications requiring verified combined cooling+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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