IFI-M · CK Sequential Integration Series

Specimen SS-F-M2-05204 // MULTI-AXIS · IFI-M

CK Sequential Integration Series IV-PR-B

Mentha × piperita

Peppermint

Tier IVCK Sequential Integration Series

Target Trait

3.3×

fold-change

Cooling+Kokumi sequential pathway fold-change

Technical Specifications

SKU

SS-F-M2-05204

Genomic Edits

10

GC Content

43.9%

Construct Length

5,192 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPM8 + CaSR

IFI Score

330

Pathway Edits

  • TRPM8 pathway primary gene cluster overexpression for cooling axis activation
  • CaSR pathway primary gene cluster overexpression for kokumi axis activation
  • Bidirectional promoter system for synchronized C+K dual-axis co-expression
  • Metabolic flux partitioning between IFI-C and IFI-K pathway branches via carbon flow optimization
  • Transcription factor co-binding site engineering for Cooling-Kokumi combinatorial gene regulation
  • Cross-pathway feedback inhibition circuit removal for C+K simultaneous accumulation
  • Cofactor balance optimization for C+K dual-pathway operation
  • Protein scaffold engineering for C+K enzyme co-localization

Key Interventions

Dual-receptor co-activation via synchronized TRPM8 and CaSR pathway engineeringMetabolic flux partitioning strategy enabling simultaneous cooling and kokumi accumulation without competing pathway suppressionSequential construct stacking for CK multi-axis integrationPEG-mediated co-transfection of axis constructs for rapid phenotypic screening of multi-axis combinations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV CK dual-axis sequential integration platform for food ingredient applications requiring verified combined cooling+kokumi sensory character. Suitable for multi-modal flavor architecture, premium ingredient certification, and receptor-confirmed IFI multi-axis profiling.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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